a procedure referred to as the protein truncation test (PTT) 4,5. We have modified this method by designing 5′ primers that include sequence coding for an epitope tag 5′ to and in frame with ...

To use the PP7 coat protein as a tag for imaging, Chao made a truncation that interfered with assembly of the capsid but still allowed the protein to dimerize and bind RNA. After the biochemical ...