Do not dilute the culture with water. Air dry thoroughly. Make certain that there is no evidence of moisture on the slide when you proceed to the next step. DO NOT HEAT FIX! Flood the slide with 1% ...
Be sure to keep the two drops separate. 4. Air dry - DO NOT HEAT FIX. 5. Flood the slide with acid-alcohol (95% ethanol, 3% HCl) until it turns blue. This generally takes ~ 2 seconds. Drain the excess ...
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