Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules to be separated are pushed by an ...
In this form of pulsed-field gel electrophoresis (transverse alternating field electrophoresis, also known as TAFE), electrodes are positioned on opposite sides of a vertical gel 1. The DNA moves ...
you will elute the insert from the agarose. More background information about agarose gel electrophoresis and related techniques (e.g., blotting) can be found at these links. Detection of DNA on the ...
and does not require post-electrophoresis staining for fast and convenient results. Both the DNA and RNA systems are also suitable for downstream applications such as qPCR or sequencing. For DNA ...
For a 30-year-old experimental method, gel electrophoresis remains popular. Originally devised as a method to extract nucleic acids from solution, it has evolved into a method for the analysis of ...