However, Guo et al. measure viability with a “live/dead” stain, a combination of calcein AM and ethidium homodimer. Calcein AM is membrane-permeable and is converted to fluorescent calcein by ...
For the endpoint assay, the spheroids were stained with a mixture of the Hoechst nuclear stain and viability dyes Calcein AM and EtHD. The spheroids were then subjected to imaging and analysis for ...
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AZoLifeSciences on MSNCell Culture Systems for Automating Cell Culture of 3D Cancer Spheroid AssaysD cancer cell models, like tumoroids, are tools of great value for cancer research and drug development because they are highly representative of the structure and behavior of tumors. Manual 3D cell ...
It is used for measuring eg TRAP stained slides or dual calcein labels on mouse bone sections. The Bioquant Osteo system has an automatic microscope stage for accurate tiling across many slide fields.
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