DNA must meet the following parameters: 260/280 of 1.70 to 2.0 Digestion of an aliquot of BAC DNA with a frequent cutter, e.g. BamHI, and run on a 0.8% agarose gel. A distinct, predicted banding ...
There is an EcoRI site within the merApe9 gene. Two EcoRI, and one each of BamHI and HindIII (neither one occurring within the gene) would be expected for a transgenic plant with a single copy of ...
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