Holy Cross is a city in Yukon-Koyukuk Census Area, Alaska, United States. At the 2010 census the population was 178, down from 227 i…Holy Cross is a city in Yukon-Koyukuk Census Area, Alaska, United States. At the 2010 census the population was 178, down from 227 in 2000. Holy Cross is located at 62°11′53″N 159°46′24″W / 62.19806°N 159.77333°W.